Search results for " ligand"
Article
A Closer Look at Affinity Ligands
…r vice-president of Science & Technology, Abzena illuminate the newest innovations with affinity ligands and provide a deeper understanding of the role affinity ligands play in the future of downstre…
Article
Re-use of Protein A Resin: Fouling and Economics
Its high specificity for the Fc region of antibodies is the primary reason for its dominance as the affinity ligand of choice in numerous immunological and purification applications (1). Specificity o…
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A Look at the Affinity Chromatography Landscape
Specific binding interactions between biologic molecules and immobilized affinity ligands allow for selective separations. With the advent of next-generation biopharmaceuticals, chromatography materia…
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Real-Time Characterization of Biotherapeutics and Comparability of Biosimilars
SPR is a rapidly emerging tool for studying ligand binding interactions with membrane proteins, which are the major molecular targets for biopharmaceutical products (6). In SPR, one of the interactant…
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Enhancing Resins Addresses Purification Concerns
These tails reversibly bind to a poly(T) ligand that can be immobilized to a solid support, which would enable an affinity-type of purification. “This is why oligo(dT) support is often used in the pur…
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Innovative Chromatography Resins Can Improve Purity and Quality
“Coupling a protein-specific affinity ligand to a suitable chromatography bead creates the corresponding affinity resin. The underlying affinity ligand molecule plays a critical role in the performanc…
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Improving Process-Scale Chromatography
New resin chemistries
New chromatography resins and chromatography ligands are constantly being developed, according to Lotta Ljungqvist, head of bioprocess R&D for Cytiva. She points to several n…
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Process Chromatography: Continuous Optimization
“More specifically, protein A resins have affinity ligands on their surfaces that have the appropriate shape and chemical substituents to allow for a three-dimensional steric fit with the Fc portion o…
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Development of Purification for Challenging Fc-Fusion Proteins
Residual ProA levels were measured by enzyme-linked immunosorbent assay (ELISA) method for the quantitation of ProA ligand in solution (Repligen). Quantitation was achieved by comparing the signal of …
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Comparing Protein A Resins for Monoclonal Antibody Purification
Protein A is the affinity chromatography ligand of choice for the first antibody capture step, because its high selectivity gives excellent purity (typically > 99%) and high yields. Furthermore, Prote…